Universal positive probes and negative control probes are needed to run with human or mouse control slides for a quality control check of the technique and with your sample for the sample or RNA quality control check.ACD provides universal positive control probes that target the Nuclear RNA U6 gene. Negative control probes target a universal scramble sequence.The detected expression level of your target RNA may vary from the positive control expression level because Nuclear U6 expression level is very high.

The manual positive control probe (Cat. No. 727871-S1) and negative control probe (Cat. No. 727881-S1) in channel S1 can be used for the miRNAscope™ HD RED assay whereas the LS positive probe (Cat. No. 727878-S1) and negative control probe (Cat. No. 727888-S1) in LS channel S1 can be used for the miRNAscope™ LS RED assay. The names for these probes do not contain a S1 or Channel S1 in its names.