Control Probes for Manual Assays: RNAscope 2.5 HD Duplex Reagent Kit

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in brain FFPE tissue.

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in brain FFPE tissue.

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in colon FFPI tissue.

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in colon FFPI tissue.

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in human Hela cells.

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in human Hela cells.

RNAscope™ 2.5 HD Duplex Assay shows expression of POL2A (Green) and TBP (red) in kidney FFPE tissue

RNAscope™ 2.5 HD Duplex Assay shows expression of POL2A (Green) and TBP (red) in kidney FFPE tissue

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in kidney FFPE tissue

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in kidney FFPE tissue

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and Polr2a (red) in lung FFPE tissue

RNAscope™ 2.5 HD Duplex Assay shows expression of PPIB (Green) and Polr2a (red) in lung FFPE tissue

Species-specific positive probes and negative control probes are required to run with human or mouse control slides for the technical quality control check and with your sample for Sample or RNA quality control check. ACD provides the positive control probes that target species-specific housekeeping genes and negative control probes that target the bacteria DapB gene.

Careful selection of the right positive control is strongly recommended.  The expected expression level of your target RNA is the key criteria consider as your positive control probe should have similar expression profile.  See the overview page for details. 

When working with RNAscope™  2.5 HD Duplex chromogenic assay, you will need one premixed duplex positive control probe and one premixed duplex negative control probe.

RNAscope™ 2.5 Duplex Positive Control Probe

Human (Cat. No. 321641), Mouse (Cat. No. 321651) 

Each control probe set contains premixed and ready to use PPIB probe in channel 1 and POLR2A probe in channel 2. Probe volume is configured for 20 reactions. 

RNAscope™ 2-plex Negative Control Probe

(Cat. No. 320751)

Contains premixed probe for DapB in both channel 1 and 2. Probe volume is configured for 20 reactions.

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