St. Louis
Come meet the ACD Team at booth # 327
We'll be here from September 23 - 25
Join the RNAscope™ Workshop (WS# 082) : Single-Molecule RNA in situ Hybridization Assays: Practical Guidelines for Detecting Gene Expression in the Tissue Context
Tuesday, Sept. 25, 8:00 - 9:30am @ Room 223
Session Credits : 1.50 CEUs
RNA is a key biomarker of the dynamic gene expression changes occurring in cells and tissues. Compared to techniques that analyze bulk tissue, identifying RNA expression at the single cell level within the morphological context provides additional information, such as identifying the precise cellular and sub-cellular location of RNA, which can be highly valuable for the validation of high-throughput expression analyses. However, sensitivity and specificity are often lacking with traditional RNA in situ hybridization (ISH), which is also technically challenging and time-consuming for routine laboratory use. The RNAscope assay is a quantifiable single-cell, single-molecule ISH technology that overcomes these challenges.
This workshop will introduce histotechnologists to this highly sensitive RNA ISH platform which incorporates both simultaneous signal amplification and background suppression to achieve single-molecule visualization in tissues with high reproducibility and consistency. This technique can be performed manually or on fully automated assay systems in one day.