The RNAscope in situ hybridization (ISH) technology is a powerful method for detecting gene expression with spatial and morphological tissue context. In this webinar, we will focus on our Integrated Co-Detection Workflow (ICW), which combines RNAscope ISH technology with immunohistochemistry (IHC) or immunofluorescence (IF) procedures to detect RNA and protein in the same sample. The webinar will cover guidelines for sample preparation, pretreatment, and detection, including tips for troubleshooting.
- Overview of RNAscope technologies
- Introduction to the Integrated Co-Detection Workflow (ICW)
- Guidelines on sample preparation and pretreatment
- Guidance on assay setup and appropriate controls
- Troubleshooting tips
- Examples of RNAscope co-detection results