The RNAscope in situ hybridization (ISH) technology is a powerful method for detection of gene expression within the spatial and morphological tissue context. For this webinar, we will focus on the RNAscope Fluorescent Multiplex assay and the RNAscope Multiplex Fluorescent v2 assay workflows. The webinar will cover guidelines for sample preparation, pretreatment, and detection, including tips for troubleshooting and interpreting results. The RNAscope assays discussed during this webinar will allow for the detection of multiple gene transcripts at the single molecule level with single-cell resolution using fluorescent based detection. Please bring your questions on how to get started with the RNAscope Fluorescent Multiplex assay and the RNAscope Multiplex Fluorescent v2 assay to the webinar and our team will answer them.
- Introduction to RNAscope Fluorescent Multiplex assay based detection
- Introduction to RNAscope Multiplex Fluorescent v2 assay based detection
- Learn how to get started with RNAscope
- Guidelines on sample preparation and pretreatment
- Guidance on assay setup and appropriate controls
- Tips on fluorescent imaging
- Examples of RNAscope results
