2021 May 20 - Using Spatial Transcriptomics to Understand the Molecular Mechanisms of Memory
Kaitlin Sullivan, MSc
PhD Student, University of British Columbia, Cembrowski Lab
Watch Video:
Emily Martersteck
Field Application Scientist, Advanced Cell Diagnostics, a Bio-Techne Brand
Outline:
This webinar will present a research project that is using spatial transcriptomics to discern the molecular mechanisms underlying fear memory.
Kaitlin Sullivan of the University of British Columbia will discuss how she is using multiplexed, fluorescent RNA in situ hybridization (mFISH) based on the RNAscope technology to assess neuronal activity during the creation and recollection of fear memory.
Learning Objectives:
- The use of mFISH to spatially resolve transcriptomically unique neuronal subpopulations.
- How to computationally analyze images and quantify expression at single-cell, single-molecule resolution.
- How to pair mFISH with morphological, circuit, and functional information to get a holistic view of cell types across multiple spatial scales.
