2020 Jun 10 - ACD Re-Broadcast: Viral Pathogenesis Research: Molecular Detection with RNAscope
Jyoti Phatak-Sheldon
Associate Scientist - Applications, Advanced Cell Diagnostics
Watch Video:
Outline:
Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the etiology and pathogenesis of viral disease.
Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity and speed. Beyond this, RNAscope ISH uniquely offers molecular detection coupled with morphological context to enable visualization of the virus in different infected tissues and cell types.
In this webinar, we will demonstrate how RNAscope can be used to detect viral infection and pathogenesis, and enhance our understanding of globally important viruses, such as SARS-CoV-2.
Who should attend:
- Researchers in the field of viral pathogenesis or infectious disease
- Researchers interested in learning more about RNAscope, BaseScope, RNA ISH and spatial genomics to detect infectious diseases
Learning Objectives:
- How to detect positive single-stranded RNA viruses (Zika, MERS-CoV, hepatitis A/C/E, coronavirus) using RNAscope technology
- How to visualize the SARS-Cov-2 viral RNA using the V-nCoV2019-S probe and detect viral replication with the V-nCoV2019-S sense probe
