2019 Aug 27 - ACD Support Webinar : Using CellProfiler to Analyze Your RNAscope Images
Amber Jolly, PhD.
Image Analysis Scientist
Watch Video:
Outline:
RNAscopeTM assay is an innovative and proprietary RNA in situ hybridization (ISH) assay based on ACD’s patented technology with signal amplification and simultaneous background noise suppression. RNAscope assay delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with anatomical context in a single assay.
View the Fluorescent Assay CellProfiler Demo
View the Chromogenic Assay CellProfiler Demo
Who should attend:
- Pathologists who may or may not be familiar with RNAscope ISH
- New and existing RNAscope Assay users (manual and automated assay platforms)
- Traditional ISH users new to the RNAscope technology
- IHC users interested in RNA ISH for complementary studies
- RNAscope users interested in Open Source image analysis options
Learning Objectives:
In this webinar, we will focus on how to analyze RNAscope assay data images using the open source platform CellProfiler. We will cover the following topics:
- RNAscope technology introduction and overview
- RNAscope and BaseScopeTM data interpretation
- Scoring guidelines for RNAscope and BaseScope assays
- RNAscope assay images quality control
- CellProfiler image analysis workflow
- CellProfiler Demo
