When assessing a potential biological therapeutic candidate, several histopathological technologies are typically employed. This ensures the target expression is validated in both normal and diseased tissues before proceeding with pre-clinical studies. Protein B is a transmembrane glycoprotein that is overexpressed in numerous cancers, including triple-negative breast cancer and is often correlated with the metastatic phenotype. In this GEN webinar, we will discuss the process involved in a typical molecular pathology laboratory. Control cell lines and the RNAScope™ Assay were utilized to validate the immunohistochemistry protocol. Next, the protein B expression was confirmed in the normal human tissues, then the corresponding gene for Protein B, (Gene B mRNA) was assessed and subsequently validated using RNAscope RNA in situ hybridization technology. Studies determined high protein B expression level in the skin and mammary glands with very minimal expression in other tissues. Further studies included confocal microscopy in determining if protein B is a good candidate for therapeutic intervention.
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